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rapF [2019-06-19 11:38:32]
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rapF [2019-06-19 11:38:32]

response regulator aspartate phosphatase, anti-activator protein, controls ComA activity
Locus
BSU37460
Isoelectric point
5.18
Molecular weight
45.45 kDa
Protein length
381 aa Sequence Blast
Gene length
1146 bp Sequence Blast
Function
control of ComA activity
Product
response regulator aspartate phosphatase
Essential
no
Synonyms
ywhJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → Genetic competence
  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein phosphatases
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Response regulator aspartate phosphatase
  • Information processing → Regulation of gene expression → Quorum sensing
  • Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence

    • Gene

    Coordinates
    3,846,001 3,847,146

    The protein

    Catalyzed reaction/ biological activity

  • control of ComA activity PubMed

    • Protein family

  • RAP family (according to Swiss-Prot)

    • Domains

  • six tetratrichopeptide repeats (according to UniProt)

    • Effectors of protein activity

  • binding of PhrF to RapF results in a conformational change of RapF and prevents it from binding ComA PubMed

    • Structure

  • 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed

    • Expression and Regulation

    Operons

    Genes
    rapF-phrF
    Description
    PubMed

    Sigma factors

  • SigH: sigma factor, PubMed, in SigH regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • ComA: activation, PubMed, in ComA regulon

    • Regulation

  • PhrF accumulates at high cell density
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    Biological materials

    Mutant

  • MGNA-A035 (rapF::erm), available at the NBRP B. subtilis, Japan
  • BKE37460 (rapF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTTGCAAATCCCTCCC, downstream forward: _UP4_CTTATTCAAGGAGGAGTGAG
  • BKK37460 (rapF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTTGCAAATCCCTCCC, downstream forward: _UP4_CTTATTCAAGGAGGAGTGAG

    • References

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